Impacts of intentional mycoplasma contamination on CHO cell bioreactor cultures
نویسندگان
چکیده
منابع مشابه
Elimination of Mycoplasma Contamination in Cell Cultures
These antibiotic treatments had a high efficiency of permanent cure: MRA 64%, ciprofloxacin 77%, BM-Cyclin 84%. Resistance to mycoplasma eradication was observed in some cell cultures: BM-Cyclin 5%, ciprofloxacin 14%, MRA 22%. Nearly all resistant contaminants that could be identified belonged to the species M. arginini and M. orale. Detrimental effects of the antibiotics were seen in form of c...
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K. Yamamoto reviewed the many different procedures which have been employed in attempting to eliminate mycoplasmas from contaminated cell cultures. These have included antibiotics (tetracyclines, kanamycin, chloramphenicol, novobiocin, tylosin, and gentamicin). In addition, surface-active compounds such as triton-X or SPS, gold salts, photodynamic inactivation, and hyperimmune sera have been ut...
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A high percentage of cell lines are chronically infected with various mycoplasma species. The addition of antibiotics that are particularly effective against these contaminants to the culture medium during a limited period of time is a simple, inexpensive, and very practical approach for decontaminating cell cultures. Here, we examined the effectiveness of the new antimycoplasma compound Plasmo...
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Contamination of cell cultures and sera used for animal virus propagation with mycoplasmas represents a serious problem, especially in virology. Therefore specific control measures must be used. To achieve this we introduced PCR for the detection of mycoplasma species in cell cultures and compared its results with ELISA and microbiological culture. Seven mycoplasma species which are the most co...
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The suitability of a 16S rRNA-based mycoplasma group-specific PCR for the detection of mycoplasma contamination in cell cultures was investigated. A total of 104 cell cultures were tested by using microbiological culture, DNA fluorochrome staining, DNA-rRNA hybridization, and PCR techniques. A comparison of the results obtained with these techniques revealed agreement for 95 cell cultures. Disc...
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ژورنال
عنوان ژورنال: Biotechnology and Bioengineering
سال: 2019
ISSN: 0006-3592,1097-0290
DOI: 10.1002/bit.27161